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KMID : 0363620100310050112
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Journal of Korean Oriental Medicine
2010 Volume.31 No. 5 p.112 ~ p.123
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Stimulation of the Immune Response by Yin-Tonifying Formula
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Jung Da-Young
Ha Hye-Kyung
Lee Ho-Young
Lee Jin-Ah
Lee Jun-Kyoung
Huang Dae-Sun
Shin Hyeun-Kyoo
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Abstract
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Objectives: Three yin-tonifying formulae (Ssanghwa-tang, Yukmijihwang-tang and Jaeumganghwa-tang) were applied to investigate their immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in the murine macrophage cell line (RAW 264.7) and in ovalbumin (OVA)-immunized mice.
Methods: This study was carried out in nitricoxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes in association with three herbal formulas. C57BL/6 mice were immunized intraperitoneally with OVA/aluminum (/mouse) on days 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes) in plasma were measured.
Results: All three yin-tonifying formulas significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<0.001). Also, these herbal formulas all significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group.
Conclusion: These results suggested that the three yin-tonifying formulae could be used as stimulators of immune response.
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KEYWORD
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Ssanghwa-tang (shuanghe-tang), Yukmijihwang-tang (liuweidihuang-tang), Jaeumganghwa-tang (ziyinjianghuo-tang), immune response
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